For the collection of conformational constraints, a three-dimensional 15N-edited NOESY-HSQC and two 13C-edited NOESY-HSQC spectra for the aliphatic and aromatic regions (Muhandiram et al. 1993) were recorded with 80 ms mixing time.
Spectrum properties
Standardised name
N15NOESY
Spectrum type
N15NOESY
FID available
no
Tags
(none)
Folding/aliasing
Unspecified/other
Frequency axis definition
ID
Label
Frequency [MHz]
Range [ppm]
Data points
Source file
Database
Min.
Max.
ω1
N
N
81.099
102.574
135.199
128
ω2
HN
HN
800.350
6.283
10.784
662
ω3
H
H
800.350
-2.275
11.652
800
Experimental details
Spectrometer model
Bruker AV 800
Nominal 1H frequency
800.0 MHz
Isotope labeling
uniform 13C, uniform 15N
Sample concentration
1.100 mM
Temperature
297.00 K
Sample pH
6.00
System information
Total size (including converted files)
651.022 MB
Original format
Sparky
UUID in database
5f79a67b-4daa-43dd-9516-a79c3f9ca311
UUID on disk
d15e2dd2d45249c18b00ff335151ffa1
Spectrum projections
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